2-8°C
PRImeZOL™ Reagent is a ready-to-use reagent for the isolation of total RNA from various biological materials such as animal and plant tissues (rich in polysaccharides and proteoglycans), cell culture and bacterial cells.
This procedure is based on the sample lysis in cationic detergent guanidinium thiocyanate (GTC), followed by organic extractions and alcohol precipitation. The biological sample is homogenized or lysed before being separated into three phases: an aqueous phase (upper), an organic phase (lower) and an interphase. The RNA remains in the aqueous phase and its purification is followed by precipitation in isopropyl alcohol.
After removal of the aqueous phase, the DNA and proteins in the sample can be recovered by sequential precipitation. PRImeZOL™ Reagent contains phenol and the mixture of other reagents to ensure optimal results.
Advantages & Features:
• Quick isolation of high-quality total RNA, DNA and/or protein from a single sample
• Ready-to-use solution
• Relevant cost-saving
• Proven performance with large or small amounts of tissue or cells
• Versatile: ideal for a wide variety of biological materials such as animal and plant tissues (rich in polysaccharides and proteoglycans), cell culture and bacterial cells
• Pure RNA: ideal for any downstream application such as RT-PCR, in vitro translation, Northern blotting, RNase protection assays or dot blot hybridization
Applications:
• Purified RNA is ideal for any downstream application such as RT-PCR, in vitro translation, Northern blotting, RNase protection assays or dot blot hybridization
• Purified DNA can be used for PCR and Southern blotting
• Purified protein can be used for Western blotting
*Developed and manufactured by Canvax Biotechnology, S.L under contractual agreement for AG Scientific, Inc.
0.1 lbs
Research or further manufacturing use only, not for food or drug use.
